Neural blastocyst complementation enables mouse forebrain organogenesis
نویسندگان
چکیده
منابع مشابه
A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC).
Gene expression and binary interaction techniques are vital tools that shape our understanding of protein complexes. An inherent flaw, however, with most current protein-protein interaction techniques is the variability in expression levels for fusion proteins when using several individual plasmids. Here, we describe a novel recombination-based cloning strategy called 2in1 that enables co-expre...
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ژورنال
عنوان ژورنال: Nature
سال: 2018
ISSN: 0028-0836,1476-4687
DOI: 10.1038/s41586-018-0586-0